1 00:00:08,070 --> 00:00:04,550 hello everyone i'm 2 00:00:11,830 --> 00:00:08,080 from central superlike and 3 00:00:14,549 --> 00:00:11,840 i did my ph.d and soundtalk super like 4 00:00:15,589 --> 00:00:14,559 but now i'm a postdoc from i am a pm 5 00:00:18,950 --> 00:00:15,599 essay 6 00:00:22,390 --> 00:00:18,960 today i mainly talk about my work 7 00:00:24,870 --> 00:00:22,400 from my phd study the search for 8 00:00:26,550 --> 00:00:24,880 life biosecond nutritional mars the 9 00:00:30,870 --> 00:00:26,560 detection of dna with 10 00:00:34,630 --> 00:00:30,880 ti major thermal chemists on sam 11 00:00:35,430 --> 00:00:34,640 and they know mars has been very popular 12 00:00:38,470 --> 00:00:35,440 these days 13 00:00:41,430 --> 00:00:38,480 and we are trying to search for 14 00:00:41,750 --> 00:00:41,440 live bios like neutrals on mars and this 15 00:00:43,990 --> 00:00:41,760 is 16 00:00:44,950 --> 00:00:44,000 because the past environmental 17 00:00:48,549 --> 00:00:44,960 conditions 18 00:00:51,670 --> 00:00:48,559 on mars are like the earth today 19 00:00:54,630 --> 00:00:51,680 and this means 20 00:00:55,750 --> 00:00:54,640 the mars could be conducive to the 21 00:00:58,950 --> 00:00:55,760 emergence 22 00:00:59,750 --> 00:00:58,960 of probiotic chemistry or even the 23 00:01:02,869 --> 00:00:59,760 origin of 24 00:01:06,070 --> 00:01:02,879 life until now on mars 25 00:01:07,109 --> 00:01:06,080 they have found the perennial liquid 26 00:01:10,390 --> 00:01:07,119 water 27 00:01:13,590 --> 00:01:10,400 some organic molecules and there are 28 00:01:16,950 --> 00:01:13,600 energy sources so if it's 29 00:01:22,230 --> 00:01:16,960 possible to find some left bell 30 00:01:29,190 --> 00:01:25,749 and to answer these questions they 31 00:01:32,630 --> 00:01:29,200 need to do the insulting analysis 32 00:01:35,990 --> 00:01:32,640 by robbers they hand waking 33 00:01:40,230 --> 00:01:36,000 curiosity perseverance and 34 00:01:44,389 --> 00:01:40,240 extra mars and we also have 35 00:01:47,350 --> 00:01:44,399 the lab simulation experiments 36 00:01:50,789 --> 00:01:47,360 and the lab results will support the 37 00:01:54,630 --> 00:01:50,799 data collected by this rover 38 00:01:59,109 --> 00:01:54,640 and among these rovers they had waking 39 00:02:03,910 --> 00:01:59,119 curiosity rover and extra mars 40 00:02:07,429 --> 00:02:03,920 these three rovers they have their 41 00:02:10,790 --> 00:02:07,439 sample analysis instruments 42 00:02:14,150 --> 00:02:10,800 and by 43 00:02:17,190 --> 00:02:14,160 like sam and the moma and they can 44 00:02:22,070 --> 00:02:17,200 do the insulting analysis 45 00:02:25,030 --> 00:02:22,080 of the martian samples 46 00:02:26,309 --> 00:02:25,040 and during the institute analysis of 47 00:02:32,070 --> 00:02:26,319 martian samples 48 00:02:35,830 --> 00:02:32,080 we will apply the derivation method 49 00:02:39,110 --> 00:02:35,840 here we can say the enzyme we have 50 00:02:41,830 --> 00:02:39,120 different capacitors containing the 51 00:02:42,470 --> 00:02:41,840 relationship engines here the green 52 00:02:47,750 --> 00:02:42,480 colors 53 00:02:50,390 --> 00:02:47,760 the tmih and mtbs tfa and dmif 54 00:02:52,550 --> 00:02:50,400 and they already have done some work 55 00:02:54,390 --> 00:02:52,560 experiments with the generalization 56 00:02:58,229 --> 00:02:54,400 method 57 00:02:58,949 --> 00:02:58,239 for example in last year november we did 58 00:03:02,070 --> 00:02:58,959 the first 59 00:03:04,110 --> 00:03:02,080 tmh experiments on mars and today they 60 00:03:07,110 --> 00:03:04,120 mainly talk about the tmh 61 00:03:10,390 --> 00:03:07,120 thermochamalysis so 62 00:03:10,949 --> 00:03:10,400 what's the tmatriosomal chemolysis it's 63 00:03:14,070 --> 00:03:10,959 like 64 00:03:17,190 --> 00:03:14,080 we have non-volatile compounds 65 00:03:18,390 --> 00:03:17,200 that is this means it's very difficult 66 00:03:21,430 --> 00:03:18,400 to be detected 67 00:03:24,149 --> 00:03:21,440 by our device but if we apply 68 00:03:25,990 --> 00:03:24,159 our tmh thermal camolysis the 69 00:03:29,190 --> 00:03:26,000 non-volatile compounds 70 00:03:32,390 --> 00:03:29,200 will become volatile and 71 00:03:33,270 --> 00:03:32,400 it can be detected by our device here we 72 00:03:37,589 --> 00:03:33,280 give one 73 00:03:41,110 --> 00:03:37,599 example when we apply our tmh 74 00:03:42,229 --> 00:03:41,120 to analyze fatty acids the menstrual 75 00:03:45,190 --> 00:03:42,239 functional group 76 00:03:46,390 --> 00:03:45,200 from tmh will replace the active 77 00:03:50,390 --> 00:03:46,400 hydrogen of 78 00:03:54,789 --> 00:03:50,400 fatty acids so unless they 79 00:03:57,190 --> 00:03:54,799 will have the fatty acids derivatives 80 00:03:58,309 --> 00:03:57,200 so the during the tiamix thermal 81 00:04:02,070 --> 00:03:58,319 chemistry 82 00:04:06,470 --> 00:04:02,080 it will help to increase the mortality 83 00:04:09,670 --> 00:04:06,480 and decrease the polarity of the sample 84 00:04:12,789 --> 00:04:09,680 so we can say in this process the 85 00:04:16,229 --> 00:04:12,799 tmh summer camelisis played a very 86 00:04:20,550 --> 00:04:19,590 and as we mentioned before we are trying 87 00:04:23,909 --> 00:04:20,560 to 88 00:04:27,189 --> 00:04:23,919 search for life signatures on mars 89 00:04:29,430 --> 00:04:27,199 and we know there are so many 90 00:04:31,110 --> 00:04:29,440 important organic compounds they're 91 00:04:34,150 --> 00:04:31,120 important for life 92 00:04:37,590 --> 00:04:34,160 but for dna and on a 93 00:04:37,990 --> 00:04:37,600 they they are they are essential for 94 00:04:39,990 --> 00:04:38,000 life 95 00:04:42,710 --> 00:04:40,000 because they carry the information of 96 00:04:46,230 --> 00:04:42,720 life so they'd like to say 97 00:04:49,350 --> 00:04:46,240 if it's possible to detect the life that 98 00:04:54,469 --> 00:04:49,360 the dna from life cells 99 00:04:58,390 --> 00:04:54,479 these are tr major thermal chemists 100 00:05:01,670 --> 00:04:58,400 that have been applied on sam 101 00:05:03,590 --> 00:05:01,680 so to achieve these goals we started 102 00:05:06,550 --> 00:05:03,600 with a very simple 103 00:05:07,670 --> 00:05:06,560 chemical standards first we apply our 104 00:05:10,790 --> 00:05:07,680 tmh 105 00:05:12,310 --> 00:05:10,800 soma camilysis to analyze the seven 106 00:05:16,150 --> 00:05:12,320 nuclear bases 107 00:05:19,749 --> 00:05:16,160 the nuclear size and the nucleotides 108 00:05:23,430 --> 00:05:19,759 and then they applied a polymers here 109 00:05:26,950 --> 00:05:23,440 it's poly a because this is a tile 110 00:05:29,670 --> 00:05:26,960 way and next we applied 111 00:05:31,590 --> 00:05:29,680 the tia major thermocouples to analyze 112 00:05:34,230 --> 00:05:31,600 the dna fragments 113 00:05:35,110 --> 00:05:34,240 from the life cells here we choose e 114 00:05:42,230 --> 00:05:35,120 coli 115 00:05:44,870 --> 00:05:42,240 and very easier to get it 116 00:05:45,270 --> 00:05:44,880 so all these experiments they have been 117 00:05:48,909 --> 00:05:45,280 done 118 00:05:50,830 --> 00:05:48,919 with the pyrolysis and against 119 00:05:54,550 --> 00:05:50,840 chromatography and the mass 120 00:05:58,629 --> 00:05:54,560 spectrometer this device 121 00:06:01,990 --> 00:05:58,639 has the same mechanism 122 00:06:05,590 --> 00:06:02,000 with some instrument so you can apply 123 00:06:10,469 --> 00:06:05,600 the pyrgcms to analyze 124 00:06:13,909 --> 00:06:10,479 to do the simulation experiments in lab 125 00:06:17,189 --> 00:06:13,919 first like make the the 126 00:06:20,710 --> 00:06:17,199 nuclear bases with tmj summer camalysis 127 00:06:22,230 --> 00:06:20,720 experiments we only take the anthony as 128 00:06:25,270 --> 00:06:22,240 an example 129 00:06:28,550 --> 00:06:25,280 so when we apply tmh to 130 00:06:31,430 --> 00:06:28,560 analyze the nuclear the antonine 131 00:06:32,629 --> 00:06:31,440 we can say the messel functional group 132 00:06:35,909 --> 00:06:32,639 from tmh 133 00:06:37,510 --> 00:06:35,919 will replace the active hydrogen from 134 00:06:41,510 --> 00:06:37,520 adenine 135 00:06:45,590 --> 00:06:41,520 so they have different derivatized 136 00:06:48,309 --> 00:06:45,600 adenine from the result they can say 137 00:06:51,909 --> 00:06:48,319 they have different peaks 138 00:06:55,909 --> 00:06:51,919 of mesolithic anteline this is because 139 00:06:59,670 --> 00:06:55,919 for anthony they have it has isomers 140 00:07:02,629 --> 00:06:59,680 and when we apply tmh to the derivation 141 00:07:04,350 --> 00:07:02,639 we have different cases they have 142 00:07:07,510 --> 00:07:04,360 vitamin cell 143 00:07:10,070 --> 00:07:07,520 replacement and two main cell 144 00:07:12,870 --> 00:07:10,080 products and the three massive 145 00:07:17,110 --> 00:07:12,880 functional groups 146 00:07:19,589 --> 00:07:17,120 replacement so 147 00:07:20,469 --> 00:07:19,599 that's where they have different pics 148 00:07:22,950 --> 00:07:20,479 from 149 00:07:24,629 --> 00:07:22,960 uh tiana makes them a commonalist with 150 00:07:28,230 --> 00:07:24,639 anthony 151 00:07:31,909 --> 00:07:28,240 and they also compare the peak intensity 152 00:07:35,110 --> 00:07:31,919 of anthony at different temperature 153 00:07:38,150 --> 00:07:35,120 we can say with increase of temperature 154 00:07:41,589 --> 00:07:38,160 the intensity of mesolithic adenine is 155 00:07:42,950 --> 00:07:41,599 highest and 600 degrees so we say that 156 00:07:45,990 --> 00:07:42,960 600 degree 157 00:07:49,510 --> 00:07:46,000 is the optimal temperature for the 158 00:07:52,710 --> 00:07:49,520 detection of nuclear bases 159 00:07:56,070 --> 00:07:52,720 and next we analyze 160 00:07:59,189 --> 00:07:56,080 the da da mp and the atp 161 00:08:01,990 --> 00:07:59,199 with tmaj thermal camoulysis 162 00:08:04,309 --> 00:08:02,000 when they we can say from the result 163 00:08:05,830 --> 00:08:04,319 they found their typical structure the 164 00:08:09,430 --> 00:08:05,840 menstrual fulfarial 165 00:08:12,230 --> 00:08:09,440 musculated phosphate and we found the 166 00:08:12,950 --> 00:08:12,240 muscle lady nuclear bases are the main 167 00:08:17,029 --> 00:08:12,960 products 168 00:08:19,589 --> 00:08:17,039 from the these dna fragments 169 00:08:20,309 --> 00:08:19,599 and surprisingly we also found the 170 00:08:23,909 --> 00:08:20,319 message 171 00:08:27,749 --> 00:08:23,919 d a from 172 00:08:30,869 --> 00:08:27,759 d a d a m p and the atp 173 00:08:34,870 --> 00:08:30,879 with tmatr somocamolysis and this 174 00:08:40,790 --> 00:08:34,880 means our tmh can protect 175 00:08:43,430 --> 00:08:40,800 the structure of our dna fragments 176 00:08:44,949 --> 00:08:43,440 and though they analyzed the nuclear 177 00:08:48,230 --> 00:08:44,959 bases they found that 178 00:08:49,910 --> 00:08:48,240 temperature can influence the detection 179 00:08:54,790 --> 00:08:49,920 of dna fragments 180 00:08:56,630 --> 00:08:54,800 so we did we optimize the temperature 181 00:08:59,990 --> 00:08:56,640 for the detection of da 182 00:09:03,910 --> 00:09:00,000 da mp and d80p they found 183 00:09:09,509 --> 00:09:03,920 200 degrees in the optimal temperature 184 00:09:13,030 --> 00:09:09,519 for the detection of these dna fragments 185 00:09:15,670 --> 00:09:13,040 and we found that if you'd like to 186 00:09:16,310 --> 00:09:15,680 detect the phosphate the hepato should 187 00:09:20,430 --> 00:09:16,320 it be 188 00:09:21,590 --> 00:09:20,440 and 300 degrees and to detect the 189 00:09:24,710 --> 00:09:21,600 deoxyribose 190 00:09:27,190 --> 00:09:24,720 temperature should be at 600 degrees 191 00:09:28,150 --> 00:09:27,200 and for the detection of nuclear bases 192 00:09:37,910 --> 00:09:28,160 here 193 00:09:41,030 --> 00:09:37,920 we can imagine 194 00:09:41,910 --> 00:09:41,040 if we analyze the poly a at very high 195 00:09:44,470 --> 00:09:41,920 temperature 196 00:09:46,790 --> 00:09:44,480 this poly a could be decomposed to 197 00:09:50,150 --> 00:09:46,800 several parts 198 00:09:53,509 --> 00:09:50,160 but when they apply rtmh 199 00:09:56,790 --> 00:09:53,519 to analyze the poly a we found their 200 00:10:00,230 --> 00:09:56,800 typical structure of dna fragments 201 00:10:03,509 --> 00:10:00,240 and we also found the 202 00:10:06,790 --> 00:10:03,519 mesolated dna from 203 00:10:10,949 --> 00:10:06,800 poly a with tmatr summer camolysis 204 00:10:14,710 --> 00:10:10,959 so again this proved that tmh 205 00:10:17,990 --> 00:10:14,720 can protect all poly a from 206 00:10:21,030 --> 00:10:18,000 degrading and lastly 207 00:10:22,389 --> 00:10:21,040 analyze the dna fragments from the e 208 00:10:25,030 --> 00:10:22,399 coli cells 209 00:10:25,750 --> 00:10:25,040 and we can say from the result we found 210 00:10:29,030 --> 00:10:25,760 almost 211 00:10:31,750 --> 00:10:29,040 all of the nuclear bases with rtmh 212 00:10:33,350 --> 00:10:31,760 from our e-coli cells and also they 213 00:10:36,870 --> 00:10:33,360 found the 214 00:10:39,190 --> 00:10:36,880 met the typical structure of like 215 00:10:40,150 --> 00:10:39,200 the messenger phosphate medicine of a 216 00:10:42,949 --> 00:10:40,160 ferrio 217 00:10:45,190 --> 00:10:42,959 and this means or tm matrix thermal 218 00:10:48,310 --> 00:10:45,200 carbolysis can be applied to 219 00:10:53,030 --> 00:10:48,320 analyze the dna fragments from 220 00:10:56,310 --> 00:10:53,040 cells so unless we conclude that 221 00:10:57,670 --> 00:10:56,320 our ti matri semicondolysis can be used 222 00:11:00,790 --> 00:10:57,680 to 223 00:11:01,350 --> 00:11:00,800 search for life second signatures such 224 00:11:05,269 --> 00:11:01,360 as 225 00:11:09,829 --> 00:11:05,279 dna or a fragments from life cells 226 00:11:12,550 --> 00:11:09,839 but this is only the first step for the 227 00:11:13,509 --> 00:11:12,560 detection of lifestyle markers from 228 00:11:17,190 --> 00:11:13,519 sales 229 00:11:19,350 --> 00:11:17,200 and next we can analyze the bacteria 230 00:11:22,470 --> 00:11:19,360 that have been detected in emerging 231 00:11:25,350 --> 00:11:22,480 analogues that will be more interesting 232 00:11:26,550 --> 00:11:25,360 and all results will provide important 233 00:11:29,190 --> 00:11:26,560 reference data 234 00:11:31,910 --> 00:11:29,200 for the interpretation of data collected 235 00:11:34,150 --> 00:11:31,920 by the rovers 236 00:11:37,030 --> 00:11:34,160 and last i'd like to thank you for the 237 00:11:38,230 --> 00:11:37,040 support of french space agency and sam 238 00:11:41,509 --> 00:11:38,240 muma founding 239 00:11:43,190 --> 00:11:41,519 and also thanks for the support of cesar 240 00:11:46,389 --> 00:11:43,200 scholarship